Cryopreservation of Fish Gametes by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9789811540257
Publisher: Springer Singapore

7.3 What Happens when a Sperm Is Frozen and Thawed?

Simply freezing and thawing sperm or for that matter any mammalian cell in tissue fluid or compatible salt solutions, results in cell death. The lethality of freeze-thawing is apparently associated with the formation of ice rather than sub-zero temperatures since super cooling (cooling below the freezing point without ice formation) to temperatures as low as −15 °C permits survival, but freezing at this temperature and below does not. The damage to tissues during freezing and thawing is caused by:1.Internal ice crystal formation that affects the structure of cells

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